Project Information
We harvested bone marrow-derived HSPCs from 11 healthy donors at the neonate, infant, child, adult and aging stages. HSPCs were labeled with canonical surface markers representing 8 CD34 + cell populations, including hematopoietic stem cell (HSC), multipotent progentior (MPP), lymphoid-primed multipotent progenitor (LMPP), multi-lymphoid progenitor (MLP), common myeloid progenitor (CMP), granulocyte/macrophage progenitor (GMP), megakaryocyte-erythroid progenitor (MEP) and B-NK cell progenitor (B-NK).
In total, we obtained transcriptomes of 6,102 single cells to construct a temporal gene expression atlas of hematopoiesis during the human lifespan.Metadata
6,102 Cells 11 Donors 8 Immunophenotypes
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The module is to help predict cell types for single-cell RNA-seq experiments by scmap.
Users need to upload a formated file containing the gene expression values for single cells and click the Run button.
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Gene expression matrix
Matrix file contains the single-cell expression data of our 6,102 cells in scRNA-seq.
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Cell metadata
A tab-delimited file contains RNA clusters, immunophenotypes and developmental stages of each cell.
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